Vanessa de Carvalho Jovito

A Randomized Clinical Trial of Schinus terebinthifolius Mouthwash to Treat Biofilm-Induced Gingivitis.

Objectives. This study aimed to investigate the efficacy of a Schinus terebinthifolius (ST) mouthwash in reducing gingival inflammation levels (GI) and biofilm accumulation (BA) in children with gingivitis. Methods. This was a randomized, controlled, triple blind, and phase II clinical trial, with children aged 9–13 years (n = 27) presenting with biofilm-induced gingivitis. The sample was randomized into experimental (0.3125% ST, n = 14) and control (0.12% chlorhexidine/CHX, n = 13) groups. Products were masked as regards color, flavor and aroma. Intervention protocol consisted in supervised rinsing of 10 mL/day for 01 minute for 10 days. Gingival bleeding and simplified oral hygiene indexes were used to assess the efficacy variables, measured at baseline and after intervention by calibrated examiners. Data were statistically treated with paired t-test, unpaired t-test, and Wilcoxon and Mann-Whitney tests (α = .05). Results. It was found that both ST and CHX were able to significantly reduce GI levels after 10 days (P < 0.001) and there was no significant difference between them (P > 0.05). CHX was the only product able to significantly reduce BA after 10 days when compared to baseline (P < 0.05). Conclusion. ST mouthwash showed significant anti-inflammatory activity (equivalent to CHX), but it was not able to reduce biofilm accumulation.

Avaliação in vivo de Dentifrício Contendo Extrato da Eugenia uniflora L. (Pitanga) sobre Indicadores de Saúde Bucal

Objective: To assess in vivo the effect of using a dentifricevcontaining hydroalcoholic extract of the ripe fruit of Eugenia uniflora L. on the oral health i...

Eugenia uniflora Dentifrice for Treating Gingivitis in Children: Antibacterial Assay and Randomized Clinical Trial

School-age children are frequently at high risk for the onset of biofilm-dependent conditions, including dental caries and periodontal diseases. The objective of this study was to evaluate the clinical efficacy of a dentifrice containing Eugenia uniflora Linn. (Surinam cherry) extract versus a triclosan-based comparator in treating gingivitis in children aged 10-12 years. The in vitro antibacterial potential of the dentifrice was tested against oral pathogens (Streptococcus mutans, Streptococcus oralis and Lactobacillus casei). Then a phase-II clinical trial was conducted with 50 subjects aged 10-12 years, with clinical signs of gingivitis. The subjects were randomly assigned to the experimental group (n=25) and control group (n=25), in which participants used the experimental dentifrice and a triclosan-based fluoridated dentifrice (Colgate Total 12(r)), respectively. Clinical examinations assessed the presence of gingivitis (primary outcome) and biofilm accumulation (secondary outcome) using the Gingival-Bleeding Index (GBI) and Simplified Oral Hygiene Index (OHI-S), respectively, at baseline and after seven days of tooth brushing 3x/day. The data were analyzed using paired and unpaired t-test (GBI) and Wilcoxon and Mann-Whitney (OHI-S), with p≤0.05. The experimental dentifrice showed efficient antibacterial activity in vitro. In the clinical trial, a significant reduction in gingival bleeding was observed in both experimental and control groups (p<0.0001), with no statistical difference between them (p=0.178), although a small size effect was observed. Biofilm accumulation was only reduced in the control group (p=0.0039). In conclusion, E. uniflora dentifrice showed anti-gingivitis properties in children aged 10-12 years. Thus, it may be a potentially efficient and safe product to be used alternatively in preventive dental practice.

ADERÊNCIA IN VITRO DE STREPTOCOCCUS MUTANS À SUPERFÍCIE DE BRAQUETES ORTODÔNTICOS METÁLICOS E DE POLICARBONATO

Objective: to verify Streptococcus mutans (ATCC 25175) adherence to the surface of two types of orthodontic brackets. Material and Methods: Three metal and three polycarbonate brackets were used and testes according to the following groups: G1 (2 mL of BHI broth plus 140 µL of inoculum), G2 (2 mL of BHI broth containing sucrose plus 140 µL of inoculum) and G3 (2 mL of BHI broth - sterility control). A bracket of each type was immersed in each solution (G1, G2 or G3). All solutions were incubated at 37°C/24h, microaerophilic. Then, each bracket was transferred to a test-tube containing 2 mL of sterile saline solution, which underwent serial dilutions. Aliquots of 0.02 mL from each dilution were inoculated in triplicate on Petri-plates containing MSB culture medium through drop technique. Plates were incubated at 37oC, microaerophilics. After 48h, it was performed the CFU/mL counting. Results: CFU/mL means for the brackets immersed in G1 were 6.3x102 (metal) and 1x102 (polycarbonate). For G2, the means were 5.6x105 (metal) and 2.4x105 (polycarbonate). Difference was found for the number of CFU/mL adhered to the surfaces of brackets incubated in G1. Nevertheless, no difference was verified for the group incubated in G2. Conclusion: It is estimated that there are differences between metal and polycarbonate brackets’ surfaces. Presence of sucrose enhanced adherence of microorganisms to the surfaces evaluated. DESCRIPTORS: Bacterial Adhesion. Biofilms. Orthodontic Brackets.