Danilo Augusto de Holanda Ferreira
Federal University of Paraíba
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Pesquisa Brasileira em Odontopediatria e Clínica Integrada | 2009
Vanessa de Carvalho Jovito; Leopoldina de Fátima Dantas de Almeida; Danilo Augusto de Holanda Ferreira; Douglas Moura; Marçal de Queiroz Paulo; Wilton Wilney Nascimento Padilha
Objective: To assess in vivo the effect of using a dentifricevcontaining hydroalcoholic extract of the ripe fruit of Eugenia uniflora L. on the oral health i...
F1000Research | 2014
Priscila Florentino Silva; Danilo Augusto de Holanda Ferreira; Kássia Regina Simões Meira; Franklin Delano Soares Forte; Ana Maria Barros Chaves; Frederico Barbosa de Sousa
Dentin reactions to caries, crucial for pathogenesis and for the determination of the severity of caries lesions, are believed to be reasonably detected by stereomicroscopy (SM) and polarized light microscopy in quinoline (PLMQ), but accuracies are not available. Here, stereomicroscopy of wet (SW) and dry (SD) ground sections of natural occlusal caries lesions resulted in moderate (0.7, for normal dentin) and low accuracies (< 0.6, for carious and sclerotic dentin) as validated by contrast-corrected microradiography. Accuracies of PLMQ were moderate for both normal (0.71) and carious dentin (0.71). The hypothesis that detection of dentin reactions by SM and PLMQ would be influenced by the contrast quality of micrographic images was rejected. Dentin reactions were scored by SW, SD, PLMQ, and three types of microradiographic images with varying contrast qualities and each technique was compared against the one that resulted in the highest number of scores for each dentin reaction. Large differences resulted, mainly related to the detection of sclerotic dentin by both SW and SD, and normal and carious dentin by PLMQ. It is concluded that contrast-corrected microradiography should be preferred as the gold standard and SM and PLMQ should be avoided, but the relationship of PLMQ with dentin mineralization deserves further investigation.
Caries Research | 2018
Danilo Augusto de Holanda Ferreira; Carolina Patrícia Aires; Regina C. B. Q. Figueiredo; Frederico Barbosa de Sousa
The amount of organic material in the cariogenic environment correlates with the amount of organic material incorporated in carious enamel. The incorporated organic material may be expected to reduce the pore volumes available for remineralization and resin infiltration, but these expected outcomes have not yet been quantified. We tested the effect of the amount of organic content in the cariogenic agent on remineralization and the resin-occluded pore volume in artificial subsurface enamel caries. An acid gel (organic-rich; G1) and an aqueous solution (organic-poor; G2) were used to induce subsurface lesions in human enamel. Undemineralized histological sections were prepared, microradiographed, and then submitted to resin infiltration in vitro. The enamel component volumes (mineral, organic, remineralizable [total water volume], loosely and firmly bound water volumes, and resin-occluded volume) were measured (by microradiography and polarizing microscopy) at histological sites (n = 38, G1; n = 34, G2). The main outcomes were the differences between the experimental and the predicted volumes (Δremineralizable and Δresin-occluded volumes). Resin infiltration was confirmed by confocal scanning laser microscopy. Compared to G2, G1 presented more incorporated organic volume and lower Δremineralizable volume (p = 0.003; Hedges g = 0.66; power = 0.87), a lower increase in loosely bound water volume (p = 0.0013; Hedges g = 0.74; power = 0.93), a lower remineralization volume in the surface layer (p = 0.017; Hedges g = 0.68; power = 0.8), and a lower Δresin-occluded volume (p = 0.0015; Hedges g = 0.73; power = 0.92). In conclusion, the higher amount of organic matter in the cariogenic gel negatively affected remineralization and the resin-occluded volume in subsurface lesions.
Brazilian Dental Journal | 2016
Vanessa de Carvalho Jovito; Irlan de Almeida Freires; Danilo Augusto de Holanda Ferreira; Marçal de Queiroz Paulo; Ricardo Dias de Castro
School-age children are frequently at high risk for the onset of biofilm-dependent conditions, including dental caries and periodontal diseases. The objective of this study was to evaluate the clinical efficacy of a dentifrice containing Eugenia uniflora Linn. (Surinam cherry) extract versus a triclosan-based comparator in treating gingivitis in children aged 10-12 years. The in vitro antibacterial potential of the dentifrice was tested against oral pathogens (Streptococcus mutans, Streptococcus oralis and Lactobacillus casei). Then a phase-II clinical trial was conducted with 50 subjects aged 10-12 years, with clinical signs of gingivitis. The subjects were randomly assigned to the experimental group (n=25) and control group (n=25), in which participants used the experimental dentifrice and a triclosan-based fluoridated dentifrice (Colgate Total 12(r)), respectively. Clinical examinations assessed the presence of gingivitis (primary outcome) and biofilm accumulation (secondary outcome) using the Gingival-Bleeding Index (GBI) and Simplified Oral Hygiene Index (OHI-S), respectively, at baseline and after seven days of tooth brushing 3x/day. The data were analyzed using paired and unpaired t-test (GBI) and Wilcoxon and Mann-Whitney (OHI-S), with p≤0.05. The experimental dentifrice showed efficient antibacterial activity in vitro. In the clinical trial, a significant reduction in gingival bleeding was observed in both experimental and control groups (p<0.0001), with no statistical difference between them (p=0.178), although a small size effect was observed. Biofilm accumulation was only reduced in the control group (p=0.0039). In conclusion, E. uniflora dentifrice showed anti-gingivitis properties in children aged 10-12 years. Thus, it may be a potentially efficient and safe product to be used alternatively in preventive dental practice.
International Journal of Dentistry | 2010
Ricardo Dias de Castro; Irlan de Almeida Freires; Danilo Augusto de Holanda Ferreira; Vanessa de Carvalho Jovito; Marçal de Queiroz Paulo
Revista Brasileira de Ciências da Saúde | 2010
Dened Myller Barros Lima; Ricardo Dias de Castro; Irlan de Almeida Freires; Danilo Augusto de Holanda Ferreira
Revista de Iniciação Científica em Odontologia - RevICO | 2009
Vanessa de Carvalho Jovito; Leopoldina de Fátima Dantas de Almeida; Danilo Augusto de Holanda Ferreira; Irlan de Almeida Freires; Marçal de Queiroz Paulo; Ricardo Dias de Castro
Revista de Iniciação Científica em Odontologia - RevICO | 2009
Dened Myller Barros Lima; Danilo Augusto de Holanda Ferreira; Leopoldina de Fátima Dantas de Almeida; Trícia Murielly Pereira Andrade de Souza; Vanessa de Carvalho Jovito; Ricardo Dias de Castro
Rev. flum. odontol | 2009
Leopoldina de Fátima Dantas de Almeida; Danilo Augusto de Holanda Ferreira; Vanessa de Carvalho Jovito; Diana Gabriela de Sousa Soares; Ricardo Dias de Castro; Wilton Wilney Nascimento Padilha
Rev. bras. ciênc. saúde | 2009
Dened Myller Barros Lima; Ricardo Dias de Castro; Irlan de Almeida Freires; Danilo Augusto de Holanda Ferreira