Yu. A. Ushenko

NEW PARAMETER FOR DESCRIBING AND ANALYZING THE OPTICAL-ANISOTROPIC PROPERTIES OF BIOLOGICAL TISSUES

To characterize the degree of similarity inherent to parameters of the optically uniaxial birefringent protein-fibril networks of biological tissues, a new parameter — complex degree of mutual anisotropy — has been offered. The technique of polarization measuring the coordinate distributions of the complex degree of mutual anisotropy of biological tissues has been developed. It has been shown that statistical approach to the analysis of complex degree of mutual anisotropy distributions for biological tissues in various morphological and physiological states and for different optical thicknesses appears to be more sensitive and efficient in differentiation of physiological state, as compared to investigations of complex degree of mutual polarization in the corresponding laser images.

CLASSIFYING OPTICAL PROPERTIES OF SURFACE- AND BULK-SCATTERING BIOLOGICAL LAYERS WITH POLARIZATION SINGULAR STATES

The results of singular approach usage in the tasks of description and classification of appearance of optical anisotropy of different types of phase-inhomogeneous biological layers (surface-scattering, optically thin and optically thick) have been presented. The characteristic values of the fourth Stokes vector parameter (S4 = 0 — linear polarization — (L-state); S4 = ±1 — circular polarization — (±C-state)) have been chosen as the main analytical tool describing polarization-singular states. The value of S4 has been determined by the value of phase shift between the orthogonal components of amplitude in the point of biological layer laser image and therefore is azimuthally stable. Hence, statistic moments of the first to the fourth orders characterizing the distribution of the amount of characteristic values S4 = 0; S4 = ±1 have been used for definition and differentiation of optical properties of different types of biological layers — surface scattering, optically thin and optically thick human skin.

THE FEASIBILITIES OF USING THE STATISTICAL, FRACTAL AND SINGULAR PROCESSING OF HOMINAL BLOOD PLASMA PHASE IMAGES DURING THE DIAGNOSTICS AND DIFFERENTIATION OF MAMMARY GLAND PATHOLOGICAL STATES

Performed in this work are complex statistical, fractal and singular analyses of phase properties inherent to birefringence networks of protein crystals consisting of optically-thin layers prepared from blood plasma. Within the framework of a statistical approach, the authors have investigated values and ranges for changes of statistical moments of the first to the fourth orders that characterize coordinate distributions for phase shifts between orthogonal components of amplitudes inherent to laser radiation transformed by blood plasma with various pathologies. In the framework of the fractal approach, determined are the dimensions of self-similar coordinate phase distributions as well as features of transformation of logarithmic dependences for power spectra of these distributions for various types of hominal mammary gland pathologies.

Laser polarization-variable autofluorescence of the network of optically anisotropic biological tissues: Diagnostics and differentiation of early stages of cancer of cervix uteri

This research presents the results of investigation of laser polarization fluorescence of biological layers (histological sections, cytological smears) in the task of diagnostics and differentiation of early stages of cancer: Dysplasia — cervical microinvasive carcinoma of cervix uteri. The analytical conditions of polarization-optimal probing of biological layers were determined basing on the model of linear birefringence and dichroism of birefringent (fibrillar, porphyrin) networks. The technique of polarization-variable laser autofluorescence was developed and experimentally tested. The objective criteria (statistical moments) of differentiation of histological sections autofluorescent images of endometrium biopsy and cytological smears of it mucous coat were defined. The operational characteristics (sensitivity, specificity, accuracy) of this technique were determined concerning the positions of probative medicine, and clinical efficiency.

Birefringence images of polycrystalline films of human urine in early diagnostics of kidney pathology

We propose an optical model of the Mueller-matrix description of mechanisms of optical anisotropy of polycrystalline films of urine, namely, optical activity and birefringence. The algorithm of reconstruction of distributions of parameters-optical rotation angles and phase shifts of the indicated anisotropy types-are elaborated upon. The objective criteria of differentiation of urine films taken from healthy donors and albuminuria patients by means of statistical analysis of such distributions are determined. The operational characteristics (sensitivity, specificity, and accuracy) of the Mueller-matrix reconstruction method of the optical anisotropy parameters are defined.

Methods and means of Fourier-Stokes polarimetry and the spatial-frequency filtering of phase anisotropy manifestations in endometriosis diagnostics

This research presents investigation results of diagnostic efficiency of a new azimuthally stable Mueller-matrix method of laser autofluorescence coordinate distributions analysis of dried polycrystalline films of uterine cavity peritoneal fluid. A new model of generalized optical anisotropy of biological tissues protein networks is proposed in order to define the processes of laser autofluorescence. The influence of complex mechanisms of both phase anisotropy (linear birefringence and optical activity) and linear (circular) dichroism is taken into account. The interconnections between the azimuthally stable Mueller-matrix elements characterizing laser autofluorescence and different mechanisms of optical anisotropy are determined. The statistic analysis of coordinate distributions of such Mueller-matrix rotation invariants is proposed. Thereupon the quantitative criteria (statistic moments of the 1st to the 4th order) of differentiation of dried polycrystalline films of peritoneal fluid – group 1 (healthy donors) and group 2 (uterus endometriosis patients) are estimated.

Methods and means of Fourier-Stokes polarimetry and the spatial frequency filtering of phase anisotropy manifestations

The theoretical background of azimuthally stable method of Jones-matrix mapping of histological sections of biopsy of myocardium tissue on the basis of spatial frequency selection of the mechanisms of linear and circular birefringence is presented. The diagnostic application of a new correlation parameter – complex degree of mutual anisotropy – is analytically substantiated. The method of measuring coordinate distributions of complex degree of mutual anisotropy with further spatial filtration of their high- and low-frequency components is developed. The interconnections of such distributions with parameters of linear and circular birefringence of myocardium tissue histological sections are found. The comparative results of measuring the coordinate distributions of complex degree of mutual anisotropy formed by fibrillar networks of myosin fibrils of myocardium tissue of different necrotic states – dead due to coronary heart disease and acute coronary insufficiency are shown. The values and ranges of change of the statistical (moments of the 1st – 4th order) parameters of complex degree of mutual anisotropy coordinate distributions are studied. The objective criteria of differentiation of cause of death are determined.

Methods and means of Stokes-polarimetry microscopy of optically anisotropic biological layers

The results of optical modeling of biological tissues polycrystalline multilayer networks have been presented. Algorithms of reconstruction of parameter distributions were determined that describe the linear and circular birefringence. For the separation of the manifestations of these mechanisms we propose a method of space-frequency filtering. Criteria for differentiation of benign and malignant tissues of the women reproductive sphere were found.