Alexander G. Ushenko

Polarization visualization and selection of biotissue image two-layer scattering medium

We analyze and experimentally test the concept of laser polarization biotissue probing. The methods of increasing the SNR in coherent images of the optically anisotropic architectonics of the morphological biotissue structure are considered. The possibilities of polarization selection and contrasting of such images screened by other biotissues are examined. The influence of the depolarization degree of the scattered background on the SNR is investigated. The possibilities of polarization correction of the probing beam for contrasting biotissue images are analyzed.

Complex degree of mutual polarization of biological tissue coherent images for the diagnostics of their physiological state

This is the first attempt devoted to the 1st-4th order statistic analysis of the complex degree of mutual polarization of biological tissues coherent images. The ensemble of diagnostic criteria (skewness and kurtosis of two-dimensional distributions of complex degree of mutual polarization) of pathological changes of muscular and connective (skin derma) tissues is defined.

Polarization singularities of biological tissues images

The ways to the polarization singularities of the biological tissues images of various morphological structures have been theoretically analyzed. The coordinate distributions of singly (linear states of polarization) and doubly degenerated (circular states of polarization) polarization singularities of the physiologically normal and pathologically changed biological tissues have been experimentally examined. The statistical criteria of diagnostics of the kidney tissue collagenous disease (the third and the fourth statistical moments of the linear density singularity points) have been determined. It was discovered that the process of the pathological change of the kidney tissue morphology leads to the formation of the self-similar (fractal) coordinate distribution of the polarization singularities of its image.

Investigation of the correlation structure of biological tissue polarization images during the diagnostics of their oncological changes

We have considered the theoretical background of biological tissue complex polarization analysis: (1) polarization mapping; (2) polarization-correlation mapping; (3) polarization correlometry. The technique of interferometric measurements of 2D distributions of the complex degree of mutual polarization of biological tissue images has been proposed. The totality of diagnostically urgent interconnections between the statistical and correlation structure of biological tissue polarization images and the optical-geometrical structure of their architectonics has been determined.

Investigation of 2D Mueller matrix structure of biological tissues for pre-clinical diagnostics of their pathological states

The interconnection between the geometry of biological tissue (BT) structure and their polarization properties has been studied. It has been shown that for physiologically normal BT, the polarization properties of radiation scattered on architectonic nets formed by protein fibrils have a fractal character. Pathological changes of BT architectonics are accompanied by the transformation of self-similar structure of 2D Mueller-matrix elements into stochastic and statistic ones.

Polarization-based visualization of multifractal structures for the diagnostics of pathological changes in biological tissues

The dynamics of the changes in the statistical polarization structure of coherent images of biological tissues are studied for the visualization process of their optically anisotropic (collagen) structure.

Scattering of laser radiation by multifractal biological structures

Scattering of laser radiation by structured biotissues—histological mounts of bony and muscular tissues and skin, are studied.

Polarization-correlation mapping of biological tissue coherent images

We investigate the statistical polarization parameters of biological tissue histological section images with different morphological structure. First we outline the results of polarization coordinate mapping and analysis of the statistics of the first to fourth orders of biological tissue image polarization azimuth and ellipticities. Second, we study the statistics of the first to fourth orders of coordinate distributions of the complex degree of mutual polarization (CDMP) of biological tissue images. Finally, we consider the diagnostic possibilities of investigating 2-D distributions of CDMP of images that correspond to physiologically normal and degeneratively and/or dystrophycally changed biological tissues that are being analyzed.

Laser polarimetry of pathological changes in biotissues

The possibility of using laser polarization techniques to diagnose the appearance and development of pathological changes in the morphological structure of biotissues is analyzed.

Polarization-phase mapping and reconstruction of biological tissue architectonics during diagnosis of pathological lesions

Specific features of the formation of local and statistical polarization structures of laser radiation scattered in phase-inhomogeneous layers (PIL) of biological tissue (BT) were studied. The distribution of azimuth and eccentricity of boundary field polarization was found to correlate with the orientation-phase structure of multifractal PIL. A method of polarization phase reconstruction of BT architectonics was suggested.