Alice Eiko Murakami

Fatty acid profile of egg yolk lipids from hens fed diets rich in n-3 fatty acids

Abstract The effects of diet and of strain on lipid, cholesterol and fatty acid compositions of egg yolk were studied. Two hen strains (Red Lohman—RL and White Lohman—WL) and four experimental diets with different fatty acid composition were used. No significant (P 0.05) by the experimental diets. The major effects of the diets were observed in fatty acids C16:0, C18:0, C18:1n9, C18:2n6, C20:4n6, C20:5n3 and C22:6n3. The addition of oils to the diets fed to hens allowed the production of eggs with higher n3/n6 and PUFA/SFA fatty acid ratios than the eggs from control hens. It was concluded that the amounts of saturated and unsaturated fatty acids in egg yolk could be altered by dietary manipulation.

Effect of arginine on the development of the pectoralis muscle and the diameter and the protein:deoxyribonucleic acid rate of its skeletal myofibers in broilers

This work aimed at evaluating the effects of the supplementation of starter diet with Arg on breast muscle development in broilers and the activation of satellite cells and the aggregation of myofibrillar protein. Male Cobb chicks (n = 990) were randomly assigned to 1 of 5 treatments in a complete random design. Measurements of 33 chicks per treatment were made in 6 repetitions. The treatments consisted of a basal diet with 1.390% digestible Arg (without supplementation) and 4 dietary levels of Arg (1.490, 1.590, 1.690, and 1.790%) with Arg:Lys ratios of 1.103, 1.183, 1.262, 1.341, and 1.421, respectively. Arginine supplementation was used only in the starter phase (1 to 21 d). Dietary supplementation with Arg had a positive effect (P < 0.05) on breast and breast fillet weight on d 7 and 21 and on myofiber diameter on d 14 and 21. However, no effect was observed (P > 0.05) on the protein:DNA ratio, which demonstrates that Arg does not interfere with the mitotic activity of the satellite cells. Independently from mechanism, Arg affected muscle growth in the starter phase positively. Dietary supplementation with Arg in the starter phase had no effect (P > 0.05) on the carcass yield of broilers on d 42. Diet supplementation with Arg at levels above the ones recommended for the starter phase may be necessary for improved muscle development in broilers.

Influence of glutamine and vitamin E on the performance and the immune responses of broiler chickens

This study aimed at evaluating the influence of Glutamine (Gln) and Vitamin E (VE) supplementation on the performance and immune response of broilers. A completely randomizes experimental design with a 2 x 3 (VE x Gln) factorial arrangement was used. VE was supplemented at 10 and 500 mg/kg feed, with or without Gln (1%) addition, and two periods of supplementation in the starter diets (1-7 and 1-14 days of age), with five replicates of 50 birds each. The analyzed parameters were: live performance (weight gain, feed intake, and feed conversion ratio); relative weights of the spleen, bursa, and thymus; antibody titers (with sheep red blood cells suspension - SRBC) and cutaneous basophilic hypersensivity (CBH). Data were submitted to the analysis of variance, and means were compared using the test of Tukey. Treatments did not influence (P>0.05) live performance parameters or antibody titers. VE reduced (P=0.01) CBH, with the level of 10 mg VE/kg allowing higher cell proliferation as compared to 500 mg VE/kg. As to lymphoid organs, only the spleen was affected (P=0.035) by Gln, which resulted in higher spleen relative weight when fed during the first week of age. Results showed that 10 mg VE/kg with Gln (1-7 days) promoted better immune responses.

Utilização do farelo de girassol na alimentação de frangos de corte

A trial was carried out to evaluate the broiler chicks performance fed with increasing sunflower meal levels. A total of 1200 one-day-old chicks were allotted in a completely randomized design with six treatments, four replicates and 50 birds by experimental unit. The treatments consisted on a corn, soybean meal based control diet (RT) and five others with 10, 20, 30, 40 and 50% of soybean meal protein replacement by sunflower meal protein. A quadratic effect of the sunflower meal levels on feed gain at initial phase, on feed intake and average weight gain at growing phase and on weight gain at total period were observed. In isoenergetic and isoaminoacid diets for digestible methionine+cystine and lysine, the soybean meal protein can be replaced by the sunflower meal protein up to 30% level, which corresponds to nearly 15% of sunflower meal inclusion in the broiler chicks diets.

Influência de fatores antinutricionais da leucena (Leucaena leucocephala e Leucaena cunningan) e do feijão guandu (Cajanus cajan) sobre o epitélio intestinal e o desempenho de frangos de corte

A trial was conducted to evaluate the influence of antinutritional factors in some feedstuffs on broiler chicks performance and intestinal epithelium. A total of 480 one-day old broiler chicks were allotted to a completely randomized experimental design with four treatments (A= corn and soybean meal based control diet; B = diet containing Leucaena leucocephala - 6% [LL]; C = diet containing Leucaena cunningan - 6% [LC] and D = diet containing Pigeous bean - Cajanus cajan - 20% [CC]), four replicates and 30 broiler chicks per experimental unit. The broiler chicks, which were fed with LL and LC containing diets showed lower weight gain and a worse feed: gain in the periods from 1 to 21 and from 1 to 42 days of age. At 21st days of age only the broiler chicks fed with LC containing diet showed a higher villi size in jejunum and villi: crypt ratio in jejunum and ileum. There was significant increase on the number of Goblet cells in the duodenum and jejunum in the broiler chicks of all treatments at the 21st day of age, and only broiler chicks fed with LL and LC containing diets showed an increased number of Goblet cells in the ileum. At 42th day of age, an increase of Goblet cells was observed in the duodenum of broiler chicks fed with CC containing diets, and in the ileum of broiler chicks fed with LL, LC e CC containing diets. Feed intake and crypt depth were not influenced by treatments in any of the experimental periods. Alterations on jejunum villi morphology, villi: crypt ratio and increased number of Goblet cells in the initial period resulted on a worse weight gain and feed: gain in the initial and total growth periods of the broiler chicks.

Efeito da suplementação enzimática em rações com farelo de soja e soja integral extrusada sobre a digestibilidade de nutrientes, o fluxo de nutrientes na digesta ileal e o desempenho de frangos

Tres experimentos foram realizados com o objetivo de avaliar o efeito da suplementacao enzimatica em racoes a base de milho e soja sobre o desempenho (Experimento 1), a digestibilidade de nutrientes (Experimento 2) e o desempenho e o fluxo de nutrientes na digesta ileal (Experimento 3) em frangos de corte. No Experimento 1, para o calculo das racoes experimentais, a composicao quimica do farelo de soja (FS) e da soja integral extrusada (SIE) foi superestimada em 7% para os valores de EM e PB e 5% para Met, Met+Cis e Lis (Trat. C, D e E) e 9% para EM, 7% para PB e 5% para Met, Met+Cis e Lis (Trat. F, G e H), quando houve a adicao de enzimas (Allzyme Vegproâ e Allzyme Lipaseâ). Conclui-se que os valores de EM, PB e aminoacidos (Met, Met+Cis e Lis) da soja puderam ser superestimados em 9, 7 e 5%, respectivamente, quando houve a adicao de complexo multienzimatico (Allzyme Vegproâ) as racoes para frangos de corte (1-42 dias), sem prejuizo ao desempenho das aves. Os tratamentos utilizados nos experimentos 2 e 3 foram: A e B - racoes contendo farelo de soja (FS), sem e com adicao de complexo multienzimatico (Allzyme Vegproâ); e C e D - racoes contendo farelo de soja (FS) e soja integral extrusada (SIE), sem e com adicao de complexo multienzimatico (Allzyme Vegproâ). No experimento 2, nao foram observadas diferencas entre os tratamentos para os coeficientes de metabolizacao aparente de materia seca, proteina bruta, energia bruta e a EMAn. No Experimento 3, para os periodos inicial (1-21 dias de idade), de crescimento (22-42 dias de idade) e total (1-42 dias de idade), nao foram observadas diferencas no consumo de racao, no ganho de peso e na conversao alimentar das aves que receberam racoes suplementadas com enzimas. Na determinacao do fluxo de nutrientes na digesta ileal, nao foram verificadas diferencas entre os tratamentos estudados, aos 21 e 42 dias de idade.Tres experimentos foram realizados com o objetivo de avaliar o efeito da suplementacao enzimatica em racoes a base de milho e soja sobre o desempenho (Experimento 1), a digestibilidade de nutrientes (Experimento 2) e o desempenho e o fluxo de nutrientes na digesta ileal (Experimento 3) em frangos de corte. No Experimento 1, para o calculo das racoes experimentais, a composicao quimica do farelo de soja (FS) e da soja integral extrusada (SIE) foi superestimada em 7% para os valores de EM e PB e 5% para Met, Met+Cis e Lis (Trat. C, D e E) e 9% para EM, 7% para PB e 5% para Met, Met+Cis e Lis (Trat. F, G e H), quando houve a adicao de enzimas (Allzyme Vegproâ e Allzyme Lipaseâ). Conclui-se que os valores de EM, PB e aminoacidos (Met, Met+Cis e Lis) da soja puderam ser superestimados em 9, 7 e 5%, respectivamente, quando houve a adicao de complexo multienzimatico (Allzyme Vegproâ) as racoes para frangos de corte (1-42 dias), sem prejuizo ao desempenho das aves. Os tratamentos utilizados nos experimentos 2 e 3 foram: A e B - racoes contendo farelo de soja (FS), sem e com adicao de complexo multienzimatico (Allzyme Vegproâ); e C e D - racoes contendo farelo de soja (FS) e soja integral extrusada (SIE), sem e com adicao de complexo multienzimatico (Allzyme Vegproâ). No experimento 2, nao foram observadas diferencas entre os tratamentos para os coeficientes de metabolizacao aparente de materia seca, proteina bruta, energia bruta e a EMAn. No Experimento 3, para os periodos inicial (1-21 dias de idade), de crescimento (22-42 dias de idade) e total (1-42 dias de idade), nao foram observadas diferencas no consumo de racao, no ganho de peso e na conversao alimentar das aves que receberam racoes suplementadas com enzimas. Na determinacao do fluxo de nutrientes na digesta ileal, nao foram verificadas diferencas entre os tratamentos estudados, aos 21 e 42 dias de idade.

Supplemental glycine and threonine effects on performance, intestinal mucosa development, and nutrient utilization of growing broiler chickens

A study was conducted to evaluate Gly requirements in low-CP diets with different levels of digestible (dig) Thr, and their effects on performance, intestinal mucosal development, and nutrient utilization of broiler chickens from 21 to 35 d age. A total of 240 twenty-one-day-old Cobb-Vantress male broiler chickens were distributed in a completely randomized 4 × 2 factorial arrangement for a total of 8 treatments with 5 replicates of 6 birds each. The treatments consisted of 4 levels of Gly+Ser (1.47, 1.57, 1.67, or 1.77%) and 2 levels of dig Thr (0.70 or 0.77%, corresponding to 100 or 110% of Thr requirements, respectively). Common diets were fed to broilers until 20 d of age. At d 35, an interaction (P ≤ 0.01) was observed between the Gly+Ser and dig Thr levels for G:F. Glycine supplementation resulted in a linear increase (P < 0.05) in BW gain, G:F, intestinal mucin secretion, apparent digestibility of fat, and AME values of the experimental diets. Threonine levels greater than the levels required (0.77%) improved (P < 0.05) G:F and increased (P < 0.05) intestinal mucin secretion. However, intestinal morphometry and the number of goblet cells in the duodenum, jejunum, and ileum were not affected by the treatments. The dietary Gly+Ser level necessary to optimize G:F in low-CP diets containing 0.77% Thr for broiler chickens during growth was estimated to be 1.54%; however, this requirement may be greater than 1.77% in diets with 0.70% Thr. Supplemental Gly may be essential to support maximum performance for broiler chickens from 21 to 35 d of age when they are fed diets based exclusively on vegetable ingredients and with low protein levels. Glycine can directly or indirectly influence the proper function of the intestinal mucosa and improve dietary energy utilization.

Effects of organic mineral dietary supplementation on production performance and egg quality of white layers

This trial aimed at evaluating the effect of organic trace mineral supplementation of commercial layer diets on productive performance and egg quality. One-hundred-ninety-two Hy Line W36 white 69-w-old layers were distributed into a completely randomized design with three treatments, and eight replicates, with eight birds each. Treatments consisted of a basal diet supplemented with inorganic trace minerals (R1), and two others experimental diets containing 0.250 ppm (R2) and 0.500 ppm (R3) of an organic source of zinc, manganese, and selenium. Feed intake (g/bird/day), feed conversion ratio (kg/dozen egg and kg/kg egg), egg weight (g), egg production (%), thin and cracked eggshells (%), specific gravity (g/mL), Haugh Units, total egg solids (%), yolk yield, white and shell yields (%), eggshell thickness, and egg Se content were evaluated Tukeys test analyzed differences among means at 5% of probability using PROC GLM in SAS (2000). Although not significant as compared to the non-supplemented diet, improvements on relative cracked-plus-thin shells were observed with the use of organic mineral blend. The addition of the organic blend to the diet at 0.250 kg/ton resulted in (p<0.05) higher total egg solids. Also, as compared to eggs from control group, fresh and dried yolk yields were higher with the dietary inclusion of the organic mineral blend at 0.250 and 0.500 kg/ton.

Influence of Delayed Placement and Dietary Lysine Levels on Small Intestine Morphometrics and Performance of Broilers

This experiment studied the influence of delayed placement (HI) and digestible lysine level (DL) on the morphometrics of the intestinal mucosa and on the performance of broilers. A total number of 1,705 Cobb 500 male chicks were used in a completely randomized experimental design in a factorial arrangement with four HI (12, 24, 36 and 48h), and two DL level in the starter diet (1.143 and 1.267%), with four replicates and 55 birds per experimental unit. The amino acids methionine-cystine, threonine, and tryptophan were balanced according to the ideal protein (IP) concept. Small intestine morphometrics was evaluated using histology slides of the duodenum and jejunum. There was no interaction between HI and DL levels for any of the studied parameters. The 1.143% level of DL promoted better performance results at 21 and 42 days of age, as well as higher duodenum and jejunum crypt depth, and duodenum villi height at 21 days of age. HI negatively influenced the morphometrics of the small intestine during the starter phase, and the performance of broilers up to 42 days of age. There was no effect of the treatments on yolk sac utilization or abdominal fat percentage. It was concluded that the use of 1.143% DL and HI of 12 hours promoted better development of the small intestine mucosa up to 21 days of age, and broiler performance at market age.

Exigências nutricionais de cálcio e fósforo de codornas de corte em crescimento

Three experiments were carried out to estimate the calcium and phosphorus requirements of meat quail. In the first experiment (1-14 days of age), 1,250 meat quails were placed in a 5 × 5 factorial arrangement (calcium levels = 0.65, 0.76, 0.87, 0.98 and 1.09% × phosphorus levels = 0.12, 0.22, 0.32, 0.42 and 0.52%), totaling 25 treatments, with two replications of 25 birds per experimental unit. The different calcium levels did not affect bird performance. Body weight, weight gain and optic density were influenced in a quadratic form by phosphorus levels and the phosphorus requirement was estimated at 0.41%. The levels of 0.65% calcium and 0.41% phosphorus in diet were enough to meet the requirement of initial phase meat quail. In the second experiment (15-35 days of age), 1,500 meat quails were placed in a 5 × 5 factorial arrangement (calcium levels = 0.61, 0.71, 0.81, 0.91 and 1.01% × phosphorus levels = 0.29, 0.34, 0.39, 0.44 and 0.49%), totaling 25 treatments, with two replications of 30 birds per experimental unit. Differences were not observed of the calcium and phosphorus levels on bird performance. Optic density was influenced in a quadratic form by phosphorus levels and the phosphorus requirement was estimated at 0.41%. In the third experiment, to assess the calcium and phosphorus balance (28-35 days of age), a linear effect was observed on the calcium intake and excretion with the increase in the calcium levels in the diets. The levels of 0.61% calcium and 0.41% phosphorus in the diet were enough to meet the requirement of finishing meat quail. The calcium levels did not affect bird performance at 1-14 and 15-35 days of age, showing, respectively, 0.65 and 0.61% calcium levels were enough to meet the of meat quail requirement. The estimate of 0.41% phosphorus promoted performance of finishing meat quail.