Antônio Augusto de Freitas Peregrino

Up-regulation of angiotensin-converting enzyme and angiotensin II type 1 receptor in irradiated rats

Purpose: To investigate changes in cardiac functional parameters and the cardiac expression of angiotensin-converting enzyme (ACE), angiotensin II type 1 receptor (AT1), procollagen type I (proc-I) and transforming growth factor-β1 (TGF-β1) in rats irradiated at heart. Material and methods: Male Wistar rats were irradiated with a single dose of radiation (0, 5, 10 and 15 Gray [Gy]) delivered directly to the heart and the molecular evaluations were performed at various times post-irradiation (two days, 15 days and four months). The expression of ACE, AT1, proc-I and TGF-β1 were analysed using Real Time-Polymerase Chain Reaction (RT-PCR) and/or Western blotting. Cardiac structural and functional alterations were investigated at the four-month time point by echocardiography and by quantitative methods (stereology). Results: Rats irradiated with 15 Gy showed a modest reduction in the ejection fraction. Cardiac proc-I, TGF-β1, ACE and AT1 were also measurably increased. Conclusions: Irradiated rat hearts show simultaneous elevations in renin-angiotensin system components AT1 and ACE and cardiac remodeling markers proc-I and TGF-β1.

Medidas de prevenção contra câncer de pele em trabalhadores da construção civil: contribuição da enfermagem

The civil constructions workers are one of the most risk groups to the occupational cancer. The purpose was to identify these workers knowledge about skin cancer and describe protection/prevention measures adopted for them. Descriptive and qualitative nature research, developed with 50 workers of a great load building site firm at municipal district of Rio de Janeiro, Brazil, which the data was collected through a form. From descriptive and statistical analysis, the results showed that the protection measures adopted for the workers to skin cancer prevention are insufficient and the way that they are used are not lined with the literature. Important changes needed at daily of these workers are related to life styles and at access ease to informations and knowledges about skin cancer. Thus, nursings interventions along the Workers Health stuff can contribute to preventives implementation of occupational cancer.

Low-intensity infrared lasers alter actin gene expression in skin and muscle tissue

The biostimulative effect of low-intensity lasers is the basis for treatment of diseases in soft tissues. However, data about the influence of biostimulative lasers on gene expression are still scarce. The aim of this work was to evaluate the effects of low-intensity infrared lasers on the expression of actin mRNA in skin and muscle tissue. Skin and muscle tissue of Wistar rats was exposed to low-intensity infrared laser radiation at different fluences and frequencies. One and 24 hours after laser exposure, tissue samples were withdrawn for total RNA extraction, cDNA synthesis and evaluation of actin gene expression by quantitative polymerase chain reaction. The data obtained show that laser radiation alters the expression of actin mRNA differently in skin and muscle tissue of Wistar rats depending of the fluence, frequency and time after exposure. The results could be useful for laser dosimetry, as well as to justify the therapeutic protocols for treatment of diseases of skin and muscle tissues based on low-intensity infrared laser radiation.

Caspase-3 activation and increased procollagen type I in irradiated hearts

The caspase-3-cleaved presence was evaluated in this study in the heart of irradiated rats, during the decline of ventricular function. Female Wistar rats were irradiated with a single dose of radiation (15 Gy) delivered directly to the heart and the molecular, histological and physiological evaluations were performed at thirteen months post-irradiation. The expressions of procollagen type I, TGF-ß1 and caspase-3-cleaved were analyzed using Western blotting. Cardiac structural and functional alterations were investigated by echocardiography and electron microscopy. In the irradiated group, the levels of procollagen type I, TGF-ß1 and caspase-3-cleaved are increased. Significant histological changes (degeneration of heart tissue and collagen deposition) and functional (reduced ejection fraction) were observed. Data suggest that the cardiac function decline after exposure to ionizing radiation is related, in part, to increased collagen and increased caspase-3-cleaved.

Cost-effectiveness of hepatitis C treatment in slow virologic responders coinfected with HIV

Recent evidence has demonstrated that slow responders may benefit from antiviral treatment in HCV/HIV coinfection. This study aimed to evaluate the cost-effectiveness of HCV treatment in individuals with genotype 1 coinfected with HIV, with peg-interferon in combination with ribavirin, compared to the inclusion (versus non-inclusion) of slow responders. A Markov model was developed that simulated the progression of liver disease in a hypothetical cohort of one thousand men over 40 years of age, considering the Brazilian Unified National Health System (SUS) perspective and a 30-year timeline. The extension of treatment to slow responders provided a 60% increase in the number of individuals who eliminated HCV and an incremental cost-effectiveness ratio of 44,171 BRL/QALY, below the acceptability threshold proposed by World Health Organization. Sensitivity analysis did not alter the results. The inclusion of HCV/ HIV-coinfected slow virologic responders in the treatment protocol is shown to be a cost-effective strategy for the SUS.Evidencias recentes demonstram que respondedores virologicos lentos podem se beneficiar com a extensao do tratamento antiviral. O estudo investigou a adocao desse protocolo diante da coinfeccao VHC/HIV. O objetivo foi estudar a relacao de custo/efetividade da terapeutica com peguinterferon associado a ribavirina em portadores do genotipo 1 do VHC coinfectados com o HIV, comparando-se a inclusao ou nao de respondedores virologicos lentos. Simulou-se por meio de um modelo de Markov a progressao da doenca hepatica em uma coorte hipotetica de mil homens, maiores de 40 anos, considerandose a perspectiva do Sistema Unico de Saude (SUS) e horizonte temporal de 30 anos. A extensao do tratamento para respondedores lentos resultou em uma razao incremental de custo efetividade de R

Coste-efectividad del tratamiento de la hepatitis C en coinfectados con el VIH con respuesta virológica lenta

44.171/QALY, valor abaixo do limiar de aceitabilidade proposto pela Organizacao Mundial da Saude. A analise de sensibilidade nao modificou os resultados alcancados. A inclusao de individuos coinfectados VHC/HIV respondedores virologicos lentos no protocolo de tratamento apresenta-se como uma estrategia custo-efetiva para o SUS.

Custo-efetividade da inclusão dos respondedores virológicos lentos no tratamento da hepatite C na presença da coinfecção com o HIV

Recent evidence has demonstrated that slow responders may benefit from antiviral treatment in HCV/HIV coinfection. This study aimed to evaluate the cost-effectiveness of HCV treatment in individuals with genotype 1 coinfected with HIV, with peg-interferon in combination with ribavirin, compared to the inclusion (versus non-inclusion) of slow responders. A Markov model was developed that simulated the progression of liver disease in a hypothetical cohort of one thousand men over 40 years of age, considering the Brazilian Unified National Health System (SUS) perspective and a 30-year timeline. The extension of treatment to slow responders provided a 60% increase in the number of individuals who eliminated HCV and an incremental cost-effectiveness ratio of 44,171 BRL/QALY, below the acceptability threshold proposed by World Health Organization. Sensitivity analysis did not alter the results. The inclusion of HCV/ HIV-coinfected slow virologic responders in the treatment protocol is shown to be a cost-effective strategy for the SUS.Evidencias recentes demonstram que respondedores virologicos lentos podem se beneficiar com a extensao do tratamento antiviral. O estudo investigou a adocao desse protocolo diante da coinfeccao VHC/HIV. O objetivo foi estudar a relacao de custo/efetividade da terapeutica com peguinterferon associado a ribavirina em portadores do genotipo 1 do VHC coinfectados com o HIV, comparando-se a inclusao ou nao de respondedores virologicos lentos. Simulou-se por meio de um modelo de Markov a progressao da doenca hepatica em uma coorte hipotetica de mil homens, maiores de 40 anos, considerandose a perspectiva do Sistema Unico de Saude (SUS) e horizonte temporal de 30 anos. A extensao do tratamento para respondedores lentos resultou em uma razao incremental de custo efetividade de R

Building construction worker and preventive measures against skin cancer: nursing contribution

44.171/QALY, valor abaixo do limiar de aceitabilidade proposto pela Organizacao Mundial da Saude. A analise de sensibilidade nao modificou os resultados alcancados. A inclusao de individuos coinfectados VHC/HIV respondedores virologicos lentos no protocolo de tratamento apresenta-se como uma estrategia custo-efetiva para o SUS.

Trabajador de la construcción civil y las medidas de prevención contra cáncer da piel: contribución de la enfermería

Recent evidence has demonstrated that slow responders may benefit from antiviral treatment in HCV/HIV coinfection. This study aimed to evaluate the cost-effectiveness of HCV treatment in individuals with genotype 1 coinfected with HIV, with peg-interferon in combination with ribavirin, compared to the inclusion (versus non-inclusion) of slow responders. A Markov model was developed that simulated the progression of liver disease in a hypothetical cohort of one thousand men over 40 years of age, considering the Brazilian Unified National Health System (SUS) perspective and a 30-year timeline. The extension of treatment to slow responders provided a 60% increase in the number of individuals who eliminated HCV and an incremental cost-effectiveness ratio of 44,171 BRL/QALY, below the acceptability threshold proposed by World Health Organization. Sensitivity analysis did not alter the results. The inclusion of HCV/ HIV-coinfected slow virologic responders in the treatment protocol is shown to be a cost-effective strategy for the SUS.Evidencias recentes demonstram que respondedores virologicos lentos podem se beneficiar com a extensao do tratamento antiviral. O estudo investigou a adocao desse protocolo diante da coinfeccao VHC/HIV. O objetivo foi estudar a relacao de custo/efetividade da terapeutica com peguinterferon associado a ribavirina em portadores do genotipo 1 do VHC coinfectados com o HIV, comparando-se a inclusao ou nao de respondedores virologicos lentos. Simulou-se por meio de um modelo de Markov a progressao da doenca hepatica em uma coorte hipotetica de mil homens, maiores de 40 anos, considerandose a perspectiva do Sistema Unico de Saude (SUS) e horizonte temporal de 30 anos. A extensao do tratamento para respondedores lentos resultou em uma razao incremental de custo efetividade de R

DNA repair gene expression in biological tissues exposed to low-intensity infrared laser.

44.171/QALY, valor abaixo do limiar de aceitabilidade proposto pela Organizacao Mundial da Saude. A analise de sensibilidade nao modificou os resultados alcancados. A inclusao de individuos coinfectados VHC/HIV respondedores virologicos lentos no protocolo de tratamento apresenta-se como uma estrategia custo-efetiva para o SUS.