Arícia Gomes Sprada

Axial Pattern Flap from the Caudal Superficial Epigastric Artery for the Correction of Surgical Defects Created by the Resection of Tumors or Traumas in Cats and Dogs: 16 Cases (2012-2015)

The reconstruction of large skin defects originated from the excision of large tumors or trauma may require the use of reconstructive techniques given the possibility of there not being enough skin to cover the skin wound in some situations. Axial pattern flaps from the caudal superficial epigastric artery are skin flaps supplied by a large artery and, therefore, have a larger chance of survival. They are the most versatile flaps for closing defects in the caudal part of the body and may be employed to close defects on the lateral abdomen, sacrum, dorsal pelvis, base of the tail, perineum, penile sheath, inguinal region, proximal pelvic member, knee, shin and metatarsal region in cats. This study aimed at reporting 16 cases of repairs of defects originated from tumoral resection and trauma employing axial pattern flaps from the caudal superficial epigastric artery in dogs and cats.

Toxicity and oxidative stress of canine mesenchymal stromal cells from adipose tissue in different culture passages

Stem cells in regenerative therapy have received attention from researchers in recent decades. The culture of these cells allows studies about their behavior and metabolism. Thus, cell culture is the basis for cell therapy and tissue engineering researches. A major concern regarding the use of cultivated stem cell in human or veterinary clinical routine is the risk of carcinogenesis. Cellular activities require a balanced redox state. However, when there is an imbalance in this state, oxidative stress occurs. Oxidative stress contributes to cytotoxicity, which may result in cell death or genomic alterations, favoring the development of cancer cells. The aim of this study was to determine whether there are differences in the behavior of cultured mesenchymal stem cells from canine adipose tissue according to its site of collection (omentum and subcutaneous) evaluating the rate of proliferation, viability, level of oxidative stress and cytotoxicity over six passages. For this experiment, two samples of adipose tissue from subcutaneous and omentum where taken from a female dog corpse, 13 years old, Pitbull. The results showed greater levels of oxidative stress in the first and last passages of both groups, favoring cytotoxicity and cell death.

Osteossíntese biológica em tíbia de cão com aplicação de fixador esquelético externo: relato de caso

Osteossintese biologica e o tratamento dado, principalmente, as fraturas cominutivas diafisarias em quenao e possivel a reducao anatomica precisa dos fragmentos osseos, preservando o alinhamento articularsem interferir no suprimento sanguineo local. A fixacao pode ser alcancada atraves de longa ponte na areade fratura usando parafusos bloqueados, placas em ponte ou fixadores esqueleticos externos que levam acicatrizacao do tecido osseo fraturado. Demonstrou-se a utilizacao com sucesso desse principio em cao comfratura cominutiva iatrogenica da tibia causada pela colocacao equivocada do fixador esqueletico externo(FEE). A reintervencao cirurgica com a aplicacao do FEE baseando-se nos principios da osteossintese biologicapromoveu estabilidade aos fragmentos osseos envolvidos e garantiu boa cicatrizacao do tecido.

Colecistectomia videolaparoscópica em felino com colelitíase

Cholelithiasis, or gallstones, is rare in cats. This report describes the case of a six years-old mongrel cat male that had anorexia, abdominal pain, vomiting and hyperthermia. The abdominal ultrasound revealed a gallbladder with irregular shape andhyperechogenic structures, in the gallbladders neck and in the output of the cystic duct, suggesting gallstones. The patient was undergone to a laparoscopic cholecystectomy. The animal was hospitalized for a week and after sixty days the owner no longer report clinical signs previously presented.

Substituição dos ligamentos cruzados cranial e caudal em cães por duplo implante de polipropileno

The objective of this study is to point out an effective alternative in the treatment of the cranial and caudal cruciate ligaments rupture in dogs, with no association of external immobilization. Six dogs with rupture of both cruciate ligaments were included in the present study. Stifle joint was surgically stabilized by an intracapsular technique, using two polypropylene synthetic implants. The animals returned to full member support in 11.8±3.5 days and did not sustain any joint instability degree after 0, 30 and 90 days of clinical evaluation after surgery. At 90 days after surgery, it became apparent lameness in any of the six patients. It was concluded that the technique employed caused total joint stability in the absence of caudal and cranial cruciate ligaments.

Overlapping of mononuclear cells derived from bone marrow in rats' intervertebral discs: an in vitro study

Neste trabalho, foram utilizadas celulas mononucleares provenientes da medula ossea (MO) de ratos para implantacao em discos intervertebrais, a fim de estudar a sua participacao em possivel regeneracao tecidual. Essas celulas foram obtidas por centrifugacao, em gradiente de Ficoll-Paque™ PLUS, e cultivadas em frascos apropriados, por um periodo de 14 dias. Em etapa posterior, foram submetidas a marcacao celular, em que foi utilizado o marcador citoplasmatico CM-Dil, seguida de implantacao em discos intervertebrais de ratos, em um sistema de cultivo in vitro. Foram feitas avaliacoes diarias dos discos com utilizacao de um microscopio de fluorescencia, sendo constatadas alteracoes morfologicas com um formato alongado semelhante a celulas que originalmente compoem o anel fibroso. O exame histopatologico revelou a presenca de celulas mononucleares entremeadas em fibras de colageno. As alteracoes morfologicas das celulas e a presenca de celulas viaveis sao indicios de que essas celulas se integraram e proliferaram no disco intervertebral, interagindo com o microambiente deste, assumindo inclusive caracteristicas semelhantes as apresentadas por celulas-tronco mesenquimais.