Francilaine Eloise De Marchi

Production, composition and fatty acid profile of milk and butter texture of dairy cows fed ground or pelleted concentrate with sunflower and/or lignosulfonate

The objective of this study was to evaluate the milk production, composition, milk fatty acid profile, butter texture and blood parameters of Holstein cows fed corn silage and concentrate containing one of the following: ground sunflower seeds; ground sunflower seeds treated with 50 g of lignosulfonate/kg of sunflower dry matter; pelleted sunflower seeds; or ground sunflower seeds pelleted and treated with 50 g of lignosulfonate/kg of sunflower dry matter. Four lactating cows were used, each with 130±28 days in lactation and a body weight of 569±63 kg. These animals were distributed in a Latin square design with four periods of 21 days each, with 14 days of adaptation and seven days of data collection. The diets were formulated to meet nutritional requirements and had a forage:concentrate ratio of 60:40. The milk fat was lower in the pelleted treatments. The concentrations of 16:1 n-11 and trans18:1 n-9 in the milk increased, and the n-6:n-3 ratio was higher for the pelleted treatments. The firmness and adhesiveness of the butter and the blood parameters analyzed were not affected by the treatments. Addition of lignosulfonate is not effective in protecting polyunsaturated fatty acids from the ruminal biohydrogenation process, and the pelleting process has little effect on the milk fatty acid profile and can not change the butter texture.

Effect of storage on fatty acid profile of butter from cows fed whole or ground flaxseed with or without monensin

Oito vacas da raca Holandesa com 570 ± 43 kg de peso vivo e 60 ± 20 dias de lactacao foram distribuidas em delineamento quadrado latino duplo, com quatro periodos de 21 dias, para avaliar o fornecimento de linhaca inteira ou moida, com ou sem suplementacao de monensina sodica (0,02% na MS), sobre o perfil de acidos graxos da manteiga armazenada por 15 e 45 dias. O fornecimento de linhaca moida, em comparacao ao de linhaca inteira, diminuiu as porcentagens relativas de 16:0, cis7-16:1, 17:0, e cis10-17:1, mas aumentou cis9,trans11-18:2, cis3-18:3, e omega-3 na manteiga, reduzindo a porcentagem relativa de acidos graxos de cadeia media e aumentando o conteudo de acidos graxos poliinsaturados. A suplementacao com monensina aumentou as porcentagens relativas de cis9,trans11-18:2 e tendeu a aumentar a porcentagem relativa de 17:0 e diminuir acidos graxos saturados na manteiga. A manteiga das vacas que receberam monensina na dieta apresentou menor porcentagem relativa de cis6-20:4. As porcentagens relativas de cis9-16:1, cis10-17:1, 18:0, trans11-42 18:1, cis9-18:1, cis3-18:3, cis6-20:4 na manteiga armazenada por 15 dias foram maiores que naquela armazenada por 45 dias e as porcentagens relativas de cis3-20:5 tenderam a diminuir com o aumento do periodo de armazenamento. Em consequencia, as porcentagens relativas de acidos graxos saturados e de cadeia media aumentaram com o tempo de armazenamento, enquanto acidos graxos monoinsaturados e de cadeia longa diminuiram. A manteiga enriquecida com acidos graxos poliinsaturados pode ter vida de prateleira mais curta, devido ao efeito negativo do armazenamento no perfil de acidos graxos, que pode provocar oxidacao e rancidez.

Effect of abomasal or ruminal administration of citrus pulp and soybean oil on milk fatty acid profile and antioxidant properties

Soybean oil (SBO) is rich in polyunsaturated fatty acids (FA) and rumen bypass of SBO can contribute to increase the polyunsaturated FA proportion in milk fat. Citrus pulp (CPP) is a source of antioxidants but there is little information on the effects of CP administration on milk properties. This study was performed to determine the role of rumen microorganisms in the transfer of antioxidants from CPP into milk when cows receive SBO as a source of polyunsaturated FA. Four ruminally fistulated lactating Holstein cows were assigned to a 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments: (1) SBO administered in the rumen; (2) SBO infused in the abomasum; (3) SBO + CPP administered in the rumen; and (4) SBO + CPP infused in the abomasum. Product and site of administration had no effect on yield of milk components. Concentrations of total polyphenols and flavonoids, reducing power and production of conjugated diene (CD) hydroperoxides in milk were not affected by products, but infusion in the abomasum compared with administration in the rumen increased production of CD. Milk fat FA profile was not affected by products. However, cows infused in the abomasum compared with those administered in the rumen showed lower proportions of short-chain and monounsaturated FA and higher proportions of polyunsaturated, omega 3 and omega 6 FA in milk fat, which resulted in enhanced health-promoting index of milk. Administration of SBO and CPP (0.2 + 1.0 kg/d) in the rumen or the abomasum resulted in similar milk antioxidant properties, thus suggesting that the rumen microbes have little involvement in the metabolism of antioxidants from CPP.

Pelleting in Associated with Sodium Monensin Increases the Conjugated Linoleic Acids Concentration in the Milk of Dairy Cows Fed Canola Seeds

To evaluate the effects of the pelleting and the addition of sodium monensin on production, the chemical and lipid composition of milk and butter physical characteristics, 4 Holstein dairy cows (135 days of lactation) with an average milk production of 14.7 kg/d, were supplemented with a concentrate containing ground canola seeds. The cows were assigned to a 4×4 Latin square design with a 2×2 factorial arrangement of treatments: i) ground maize, soybean meal, mineral and vitamin supplements, and ground canola seeds (CG); ii) CG concentrate with 31.5 mg of monensin added per kg of dry matter (DM); iii) CG pelleted concentrate; iv) CG concentrate with monensin addition pelleted. There was no difference in milk production and composition. The addition of monensin increased milk concentration of polyunsaturated fatty acids (PUFA), the PUFA/saturated fatty acids (SFA) ratio, and omega 6. The pelleting increased the concentration of monounsaturated fatty acids, the PUFA/SFA ratio, and the omega 6/omega 3 ratio, but decreased the concentration of SFA. The association between pelleting and the addition of monensin increased the concentration of conjugated linoleic acids by 46.9%. The physical characteristics of butter were not affected by the evaluated diets. We concluded that the concentrate with 31.5 mg of monensin added per kg DM basis combined with the pelleting improves the lipid composition of milk from Holstein cows that are on pasture and supplemented with ground canola seeds, without changing the production, milk composition, and spreadability of butter.

Intake, digestibility and ruminal parameters of dairy cows fed pelleted diets and treated with lignosulfonate-containing sunflower seeds

The objective of this study was to evaluate intake, in vitro and apparent digestibility and ruminal parameters of Holstein cows that were confined and fed corn silage and concentrate containing: ground sunflower seeds (GS); ground sunflower seeds treated with 5% lignosulfonate (GSL); pelleted sunflower seeds (PS); or ground and pelleted sunflower seeds treated with lignosulfonate (PSL). Four lactating cows with 130±28 days of lactation and body weights of 569±63 kg were used. These animals were distributed in a Latin square design with four diets and four periods of 21 days each. There were no differences in the intakes (% body weight) of dry matter (DM), organic matter (OM), mineral matter (MM), crude protein (CP), neutral detergent fiber (NDF) or acid detergent fiber (ADF). The apparent digestibility of DM, OM, CP, EE, NDF, ADF and NFC were similar for all the diets, with a tendency towards decreased CP digestibility for all the pelleted diets (65.79%) compared with the non-pelleted diets (69.66%). A negative effect of lignosulfonate was observed for the in vitro digestibility (IVD) of DM, and a negative tendency was observed for the IVD of OM. Pelleting decreased the concentration of acetic acid (55.95 × 58.82 mM) in rumen fluid. There was no effect of diet on pH (6.17) or ammonia nitrogen concentration (18.19 mg/dL). Pelleting and lignosulfonate do not promote the protective effect of sunflower seed nutrients as expected, and it may be more feasible to provide ground sunflower seed.

Rumen fermentation and nutrient flow to the omasum in Holstein cows fed extruded canola seeds treated with or without lignosulfonate

Four multiparous Holstein cows averaging 548 kg of body weight and 74 d in lactation were used in a Latin square design with four 21-d experimental periods to determine effects of feeding extruded versus non-extruded canola seed, with or without 50 g/kg lignosulfonate on rumen fermentation, nutrient flow to the omasum, and degradability of dry matter (DM) and N of each diet. The DM effective degradability increased with extrusion and lignosulfonate treatment had no effect. The effective degradability of N was similar between diets. Lignosulfonate treatment of extruded versus non-extruded canola seeds decreased ruminal and total tract apparent digestibility of organic matter. The lowest apparent ruminal and highest intestinal digestibilities of protein, expressed as a percentage of N intake were observed for cows fed extruded canola seeds without lignosulfonate. Lignosulfonate treatment and extrusion had no effect on pH and concentrations of ammonia N and volatile fatty acids in the rumen. Results suggest that extruded canola seed untreated with formaldehyde may stimulate efficiency of microbial protein synthesis and is an effective means of increasing the availability of protein in the small intestine without affecting the total tract apparent digestibility of protein.