Ferreira de Oliveira

Bmoo FIBMP-I: A New Fibrinogenolytic Metalloproteinase from Bothrops moojeni Snake Venom.

A new fibrinogenolytic metalloproteinase (Bmoo FIBMP-I) was purified from Bothrops moojeni snake venom. This enzyme was isolated through a combination of three chromatographic steps (ion-exchange, molecular exclusion, and affinity chromatography). Analyses by reverse phase chromatography, followed by mass spectrometry, showed the presence of enzyme isoforms with average molecular mass of 22.8 kDa. The SDS-PAGE analyses showed a single chain of 27.6 kDa, in the presence and absence of reducing agent. The protein has a blocked N-terminal. One of the peptides obtained by enzymatic digestion of a reduced and S-alkylated isoform was completely sequenced by mass spectrometry (MS/MS). Bmoo FIBMP-I showed similarity with hemorrhagic factor and several metalloproteinases (MP). This enzyme degraded Aα-chain faster than the Bβ-chain and did not affect the γ-chain of bovine fibrinogen. The absence of proteolytic activity after treatment with EDTA, together with the observed molecular mass, led us to suggest that Bmoo FIBMP-I is a member of the P-I class of the snake venom MP family. Bmoo FIBMP-I showed pH-dependent proteolytic activity on azocasein, but was devoid of coagulant, defibrinating, or hemorrhagic activities. The kinetic parameters of proteolytic activity in azocasein were determined (V max = 0.4596 Uh−1nmol−1 ± 0.1031 and K m = 14.59 mg/mL ± 4.610).

The psychosocial rehabilitation in the alcohol and other drugs treatment: the professionals' conception

Estudio exploratorio de abordaje cualitativa cuyo objetivo fue identificar las concepciones de profesionales de un Centro de Referencia para el tratamiento de problemas relacionados con alcohol y otras drogas acerca de la rehabilitacion psicosocial. Fueron entrevistados once profesionales de nivel superior, por medio de entrevistas semi-estructuradas. El analisis de los datos fue norteada por los presupuestos de la hermeneutica dialectica, la cual origino la categoria rehabilitacion psicosocial. Los resultados evidenciaron que en la concepcion de los profesionales, la rehabilitacion psicosocial se da en la realizacion de talleres terapeuticos, actividades externas y en el establecimiento de sociedades con otras instituciones. Se concluye que el concepto de rehabilitacion psicosocial que mas se evidencia en la percepcion de esos sujetos esta asociada al modelo psiquiatrico tradicional, arraigado a la logica de la normalidad social, el problema principal a ser superado en la asistencia a los usuarios de alcohol y otras drogas.Exploratory study with qualitative approach that had as its main objective to identify the conception of the health professionals about Psychosocial Rehabilitation from a reference Center for the treatment of alcohol and other drugs related problems. The sample consisted of eleven health professionals with an academic degree, submitted to semi-structured interviews. The data analysis was made according to the hermeneutic dialectic method which originated the Psychosocial Rehabilitation category. The data demonstrated that in the professionals conception, the Psychosocial Rehabilitation is given in the realization of therapeutic workshops, outdoor activities, and the estabilishment of a partnership with other foundations. It is concluded that the concept of Psychosocial Rehabilitation that was made clear in the conception of these subjects is still associated to the traditional psychiatric model, which is attached to the logic of social normality, the main challenge to the psychosocial model of assistance to the alcohol and other drugs users.Exploratory study with qualitative approach that had as its main objective to identify the conception of the health professionals about Psychosocial Rehabilitation from a reference Center for the treatment of alcohol and other drugs related problems. The sample consisted of eleven health professionals with an academic degree, submitted to semi-structured interviews. The data analysis was made according to the hermeneutic dialectic method which originated the Psychosocial Rehabilitation category. The data demonstrated that in the professionals conception, the Psychosocial Rehabilitation is given in the realization of therapeutic workshops, outdoor activities, and the estabilishment of a partnership with other foundations. It is concluded that the concept of Psychosocial Rehabilitation that was made clear in the conception of these subjects is still associated to the traditional psychiatric model, which is attached to the logic of social normality, the main challenge to the psychosocial model of assistance to the alcohol and other drugs users.

Work in primary health care: a comprehensive mental health care

Este estudio de abordaje cualitativa tiene como objetivo cartografiar el cuidado al usuario con necessidades en lo campo de la salud mental en uma Unidad Basica de Salud, a traves de la analisis del trabajo en equipo a la luz de la integralidad de las acciones de salud. Sus partipantes son trabajadores de salud mental, de diferentes profesiones, que son parte de los procesos de trabajo en salud mental de lo servicio. La coleccion de datos fue construida mediante lo fluxograma analizador. Los resultados muestran que, en la Unidad Basica, los flujos conectivos entre los diversos trabajadores - y entre estes y los usuarios - estan a producir y proliferar varios y distintos espacios colectivos de intercâmbios, permitiendo acciones integrales de salud a traves de una comprension ampliada del proceso de salud-enfermedad mental, construida por medio de la valorizacion de las relaciones humanas y de las subjetividades involucradas en lo espacio del trabajo en salud.