Kemal Hüsnü Can Başer

Essential Oils of Calamintha pamphylica Boiss. et Heldr. subsp. pamphylica and subsp. davisii (Quezel et Contandr.) Davis

Abstract Water-distilled essential oils of two subspecies of Calamintha pamphylica namely, subsp. pamphylica and subsp. davisii, both of which are endemic taxa to Turkey, were analyzed by GC and GC/MS. In the oil of subsp. pamphylica more than fifty compounds were characterized representing 98.4% of the total components detected. Pulegone (36.16%), menthyl acetate (27.70%) and menthol (8.99%) were identified as major constituents. More than sixty compounds were characterized in the oil of subsp. davisii representing 98.3% of the components detected, with pulegone (38.21%), menthone (9.78%), menthol (9.97%) and menthyl acetate (9–33%) as main constituents.

Chemical composition and phagocyte immunomodulatory activity of Ferula iliensis essential oils

Essential oil extracts from Ferula iliensis have been used traditionally in Kazakhstan for treatment of inflammation and other illnesses. Because little is known about the biologic activity of these essential oils that contributes to their therapeutic properties, we analyzed their chemical composition and evaluated their phagocyte immunomodulatory activity. The main components of the extracted essential oils were (E)‐propenyl sec‐butyl disulfide (15.7–39.4%) and (Z)‐propenyl sec‐butyl disulfide (23.4–45.0%). Ferula essential oils stimulated [Ca2+]i mobilization in human neutrophils and activated ROS production in human neutrophils and murine bone marrow phagocytes. Activation of human neutrophil [Ca2+]i flux by Ferula essential oils was dose‐dependently inhibited by capsazepine, a TRPV1 channel antagonist, indicating that TRPV1 channels mediate this response. Furthermore, Ferula essential oils stimulated Ca2+ influx in TRPV1 channel–transfected HEK293 cells and desensitized the capsaicin‐induced response in these cells. Additional molecular modeling with known TRPV1 channel agonists suggested that the active component is likely to be (Z)‐propenyl sec‐butyl disulfide. Our results provide a cellular and molecular basis to explain at least part of the beneficial therapeutic properties of FEOs.

Modulation of Human Neutrophil Responses by the Essential Oils from Ferula akitschkensis and Their Constituents

Essential oils were obtained by hydrodistillation of the umbels+seeds and stems of Ferula akitschkensis (FAEOu/s and FAEOstm, respectively) and analyzed by gas chromatography and gas chromatography-mass spectrometry. Fifty-two compounds were identified in FAEOu/s; the primary components were sabinene, α-pinene, β-pinene, terpinen-4-ol, eremophilene, and 2-himachalen-7-ol, whereas the primary components of FAEOstm were myristicin and geranylacetone. FAEOu/s, β-pinene, sabinene, γ-terpinene, geranylacetone, isobornyl acetate, and (E)-2-nonenal stimulated [Ca(2+)]i mobilization in human neutrophils, with the most potent being geranylacetone (EC50 = 7.6 ± 1.9 μM) and isobornyl acetate 6.4 ± 1.7 (EC50 = 7.6 ± 1.9 μM). In addition, treatment of neutrophils with β-pinene, sabinene, γ-terpinene, geranylacetone, and isobornyl acetate desensitized the cells to N-formyl-Met-Leu-Phe (fMLF)- and interleukin-8 (IL-8)-induced [Ca(2+)]i flux and inhibited fMLF-induced chemotaxis. The effects of β-pinene, sabinene, γ-terpinene, geranylacetone, and isobornyl acetate on neutrophil [Ca(2+)]i flux were inhibited by transient receptor potential (TRP) channel blockers. Furthermore, the most potent compound, geranylacetone, activated Ca(2+) influx in TRPV1-transfected HEK293 cells. In contrast, myristicin inhibited neutrophil [Ca(2+)]i flux stimulated by fMLF and IL-8 and inhibited capsaicin-induced Ca(2+) influx in TRPV1-transfected HEK293 cells. These findings, as well as pharmacophore modeling of TRP agonists, suggest that geranylacetone is a TRPV1 agonist, whereas myristicin is a TRPV1 antagonist. Thus, at least part of the medicinal properties of Ferula essential oils may be due to modulatory effects on TRP channels.

Chemical composition and insecticidal activity of edible garland (Chrysanthemum coronarium L.) essential oil against the granary pest Sitophilus granarius L. (Coleoptera)

Abstract The essential oil composition and biological activities of Chrysanthemum coronarium L. from three locations in Cyprus were investigated. A total of 35 components were identified in the oils representing 86.2 ± 0.7%–93.8 ± 0.0% (n = 3) of the oils. Camphor 25.2 ± 0.1%–9.1 ± 0.3%, santolinatriene 21.7 ± 0.1%–3.6 ± 0.1%, yomogi alcohol 12.6 ± 0.1%–3.6 ± 0.1%, cis-chrysanthenyl acetate 10.7 ± 0.1%–6.8 ± 0.1% and bornyl acetate 11.0 ± 0.5%–5.7 ± 0.0% were identified as the main components. Highest DPPH scavenging and PRAP activity was observed for the Lefkoşe sample (49.59 ± 0.19% (n = 5) and 812.77 ± 4.34 AU (n = 5)). Highest phytotoxic activity against Lemna minor L. was observed for the Salamis sample (100.0%). Lefkoşe sample showed the highest insecticidal activity (97.82 ± 1.79% mortality, S. granarius). Bornyl acetate/cis-chrysanthenyl acetate/hexadecanoic acid and camphor/santolinatriene/ yomogi alcohol chemotypes of C. coronarium are proposed from Cyprus samples according to cluster analysis of the oils together with the literature data.

Chemical and Biological Diversity of the Leaf and Rhizome Volatiles of Acorus calamus L. from Turkey

Abstract Two different isolation techniques, conventional hydrodistillation (HD) and micro-steam distillation-solid-phase microextraction (MSD-SPME), have been used to analyze the volatile constituents from the leaves and rhizomes of Acorus calamus L. by gas chromatography (GC-FID) and gas chromatography coupled to mass spectrometry (GC-MS). In all the samples studied, phenylpropanoids (16.9-35.3 %) predominated by β-asarone (15.3-16.3 % in rhizomes, 23.4-31.7 % in leaves) were the main constituent. In addition to phenylpropanoids, the acorane type sesquiterpene acorenone B (7.4-16.4 %) and elemene-type sesquiterpenes, epi-isoshyobunone (3.3-7.3 %) and shyobunone (1.5-4.6 %) were detected among the major volatile constituents in the rhizomes. The monoterpenes were represented by camphor (7.5-13.9 %) and camphene (6.1-7.7 %). In the leaf, myrcene (0.3-7.1 %), limonene (1.0-5.6 %), (Z)-β-ocimene (2.9-6.1 %) were among the major constituents. Qualitative difference of the volatiles composition in the rhizomes and leaves are discussed as well as of the volatiles obtained by HD and MSD-SPME techniques are discussed. Biological activity tests resulted in moderate anti-acetylcholinesterase effect and significant cupric reducing antioxidant potential of the leaf oil, while the rhizome oil demonstrated relatively lower effects.

Phytochemicals, antioxidant, and antityrosinase activities of Achillea sivasica Çelik and Akpulat

ABSTRACT The present study is the first report on essential oil (EO) composition, phytochemicals, and biological potential of Achillea sivasica tested against free radicals, oxidative damage, and tyrosinase enzyme. Gas-Chromatography Flame Ionization Detector (GC-FID) and gas chromatography/mass spectrometry (GC/MS) analyses revealed that β-pinene (11.5%, 9.3%, and 6.7%), β-pinene (7.0%, 3.0%, and 6.9%), 1,8-cineole (18.0%, 22.1%, and 6.7%), and camphor (7.6%, 4.1%, and 9.0%) were the major constituents in the EOs from the herb, flower, and leaves, respectively. Liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) analysis of the extracts revealed the presence of caffeoylquinic acid derivatives, luteolin, apigenin, patuletin, isorhamnetin, cirsimaritin, and santin. The leaf extracts demonstrated strongest free radical scavenging, cupric reducing, lipid peroxidation inhibition, and antityrosinase activities.

Chemical Composition, Anti-Inflammatory, Antioxidant and Antimicrobial Activity of Essential Oil from Aerial Parts of Chaerophyllum aromaticum L. from Turkey

Abstract The essential oil obtained by hydrodistillation from the aerial parts of Chaerophyllum aromaticum L. was analyzed by GC and GC/MS. Anti-inflammatory activity was evaluated by lipoxygenase inhibition assay. Antioxidant activity was tested by DPPH method. Antimicrobial activity was carried out using microdilution method against seven bacteria and one fungus. The yield of light yellow-coloured essential oil was 1.1%. Eighteen compounds were identified in oil of the aerial parts representing 99.2% of the C. aromaticum oil. Sabinene (28.1%), terpinolene (16.7%) and γ-terpinene (16.1%) were characterized as the main compounds. The oil exhibited remarkable anti-inflammatory activity with an IC50 value of 63.62±1.26 μg/ml. The oil at a concentration of 20 mg/mL inhibited DPPH radical by 2.06%. The oil exhibited moderate antibacterial activity against Staphylococcus aureus ATCC 29213 (MIC: 156 μg/ml) and S.epidermidis ATCC 12228 (MIC: 625 μg/ml). The results showed that C. aromaticum essential oil was rich in monoterpene compounds and had moderate antibacterial activity against Staphylococcus strains as well as having significant anti-inflammatory activity.

Essential oil composition of Pentzia incana (Asteraceae), an important natural pasture plant in the Karoo region of South Africa

Pentzia incana is one of the most important of all natural pasture plants in the dry interior (Karoo) region of South Africa. This highly aromatic shrub is thought to be responsible for the distinctive flavour of Karoo lamb (a registered geographical indication), yet the essential oil is here characterised for the first time. Leafy twigs are traditionally chewed for relief of stomach ache. Essential oil is associated with numerous small, multicellu- lar glands. Gas chromatography and gas chromatography/mass spectrometry analysis of 17 essential oil samples from five populations showed considerable variation in both yield (0.12% to 0.88% dry weight) and composition. Of interest was the presence of one major biosynthetic group comprising yomogi alcohol (to 38.9%), artemisia alcohol (to 26.1%), artemisia ketone (to 35.0%) and artemisyl acetate (to 9.4%). High levels of fragranol (26.9%) and fragranyl acetate (27.1%) were observed only in a single specimen. Other main compounds were 1,8-cineole (to 16.7%), santolina alcohol (to 11.3%), camphor (to 47.9%), linalyl acetate (to 17.4%) and bicyclogermacrene (to 11.8%). Six of the 10 major compounds were isolated and their identities confirmed by nuclear magnetic resonance. The volatile compounds may possibly be linked to the medicinal use of P. incana, as well as the flavour profile of Karoo lamb.

Composition and potential of Tanacetum haussknechtii Bornm. Grierson as antioxidant and inhibitor of acetylcholinesterase, tyrosinase, and α-amylase enzymes

ABSTRACT The phytochemical composition of essential oils and extracts of Tanacetum haussknechtii were investigated with GC–FID–MS and LC–MS/MS techniques and evaluated against oxidation, acetylcholinesterase, tyrosinase, and α-amylase enzymes. The major volatile constituents of T. haussknectii were found to be α- and β-pinene, and borneol. Caffeoylquinic acid derivatives and flavonoids were detected in the aqueous, alcohol, and ethyl acetate extracts. In DPPH assay, the methanol extracts exhibited the highest activity. TEAC assay resulted with superiority of all methanol and the capitula ethyl acetate extract. In β-carotene bleaching assay, linoleic acid was the best protected by the ethyl acetate extract of flower. The flower oil inhibited higher acetylcholinesterase activity than the remaining extracts. The flower ethyl acetate extract was found as the most effective inhibitor of α-amylase. The herb and the leaf+stem water extracts possess highest inhibitory effect on tyrosinase.