Clarisse Simões Coelho

Proteinograma sérico de bezerras sadias, da raça holandesa, no primeiro mês pós-nascimento

Com a finalidade de estabelecer valores de referencia do proteinograma serico e avaliar o efeito do fator etario, estudaram-se amostras de soro sanguineo de 300 bezerras sadias, da raca Holandesa, no primeiro mes pos-nascimento, distribuidas por 15 grupos etarios. O teor serico de proteina total foi minimo nos animais com ate oito horas de idade, aumentando progressivamente ate o 4o dia, quando alcancou o valor maximo seguindo, entao, com pequenas oscilacoes ate o 30o dia. Os teores de albumina serica apresentaram pequenas elevacoes a partir das 24 horas de vida, sendo o aumento significativo apos 13 a 15 dias e mantendo-se ate os 30 dias de idade, quando registrou-se o valor maximo. As taxas sericas de alfaglobulina nao demonstraram variacoes significativas entre os grupos etarios. Os teores de betaglobulina serica aumentaram progressivamente ate sete a nove dias pos-nascimento, quando alcancaram um valor maximo, sendo este seguido por pequenas variacoes ate o final das observacoes. A concentracao serica de gamaglobulina que foi minima ate oito horas de idade, evoluiu com aumentos significativos ate dois dias de idade, quando atingiu um valor maximo, tendo estabilidade dos valores ate o 11o dia de idade e, na sequencia, apresentou queda progressiva das taxas ate os 30 dias. A relacao albumina:globulina evidenciou um valor maximo nos animais com ate oito horas de idade, seguido por queda significativa ate os dois dias pos-nascimento, quando passou a apresentar valores relativamente estaveis mantidos ate os 15 dias de idade, demonstrando entao tendencia a aumentos ate o final do periodo de estudo. Todos os componentes do proteinograma, com excecao da fracao alfaglobulina, apresentaram variacoes influenciadas pela idade.

Biochemical parameters to evaluate liver function of healthy female holstein calves during the first month of live

Three hundred healthy female Holstein calves were used for assess the liver function throughout the first month of life. The following serum biochemical variables were analysed: bilirrubin (total, direct and indirect) and the activities of gamma glutamyltransferase (GGT), aspartate aminotransferase (AST) and creatine kinase (CK). The influence of age on the biochemical values was also studied. The bilirrubin values had highest levels on the 1st d of life (BT-1.360mg/dl; BC-0.150mg/dl e BL-1.175mg/dl) and then showed a continuing decrease until the 30th d of life (BT-0.350mg/dl; BC-0.050mg/dl e BL-0.300mg/dl). The serum GGT activities were at they highest point between the 16th to 24th h (945.00UI/L). There was a downward trend from the 11th d (86.00UI/), presenting the lowest activity at the 30th d (24.00UI/) similar to the activities usually found in healthy adult dairy cattle. The activities of AST and CK were higher at the 1st d of life (AST- 27.50UI/L e CK- 73.00UI/L) declining from then on (AST- 19.00UI/L e CK-41.50UI/L). Ageing showed a significant influence on all the biochemical variables studied.

Influence of acepromazine on the cardiovascular actions of dobutamine in isoflurane-anesthetized horses

The influence of acepromazine (ACP) on the effectiveness of dobutamine (DBT) in increasing blood pressure during isoflurane (ISO) anesthesia was evaluated in six horses. On separate occasions, the horses were randomly assigned to receive NaCl 0.9% (Control), ACP 0.025mg kg-1 and ACP 0.05mg kg-1. The experimental treatment was administered prior to induction of anesthesia. Maintenance of anesthesia was performed under conditions of normocapnia with ISO in oxygen. Dobutamine was administered at progressively increasing infusion rates until mean arterial pressure (MAP) reached 70mmHg or until a maximum infusion rate of 5.0µg kg-1 min-1. Compared with baseline, DBT increased heart rate, systolic, diastolic and mean blood pressures in all treatments. However, these variables did not differ among treatments. The target MAP (70mmHg) was not reached in 2/6, 2/5 and 0/6 horses in the Control, ACP0.025 and ACP0.05 treatments, respectively. The mean dose of DBT to achieve target MAP was 3.5±1.8, 3.7±1.6 and 2.7±1.4µg kg-1 min-1 in the Control, ACP0.025 and ACP0.05 treatments, respectively (P>0.05). Under the conditions of this study, premedication with ACP does not interfere with the effectiveness of DBT in increasing blood pressure in horses anesthetized with ISO.

Revisão de 26 casos clínicos de duodeno-jejunite proximal em eqüinos (1996-2000)

Medical records of 26 horses admitted to the Veterinary Hospital of FMVZ-USP from December 1996 to November 2000 with duodenitis-proximal jejunitis (DPJ) were reviewed. From 1555 horses examined during this period, 205 (13.2%) had shown gastro-intestinal problems and 1.7% of the total had DPJ. Ages, physical parameters, laboratory values and clinical course were compared among surviving horses DPJ (group 1) and horses not surviving DPJ (group 2). Twenty (76.9%) of 26 horses survived. All horses were managed with medical treatment only. The results were analysed through Student test (a=0.05). Significative differences between the two groups were observed for white blood cell counts (WBC), serum creatinine and heart rate. Laminitis was the most common complication and occurred in 30.8% of the cases.

Measurement of Incisor Overjet and Physiological Diastemata Parameters in Quarter Horse Foals.

Cephalometric studies are important to quantify abnormalities of jaw length and positioning. In this study, 4 to 7-month-old Quarter horse foals (n = 51) were examined to determine overjet (horizontal overlap) prevalence and measure the size of the physiological diastemata. Results were analyzed in relation to age, sex, and lineage. Another aim of this study was to develop a simple field technique for measuring incisor malocclusion and physiological diastemata dimensions that could be used to monitor the growth of the rostral components of maxilla, incisive bone, and mandible. The overall prevalence of overjet lesions in these foals was 51%. Females were overrepresented (61.5%). Overjet occurred more commonly in show foals (50% prevalence) than other working (7.7%) and race (42.3%) lineage foals. Significant differences were found between maxillary and mandibular physiological diastemata lengths in foals of all ages and, as expected, there was a positive statistical correlation between age and maxillary and mandibular physiological diastemata measurements. Incisor overjet was present in 44.4% of 4-month-old foals, 45.5% of 5-month-old foals, 58.3% of 6-month-old foals, and 60% of 7-month-old foals. There was a weak positive correlation between age and the presence of incisor overjet. It was concluded that incisor overjet was common among Quarter horse foals, especially those from show and race lineages. The field technique for physiological diastema measurements was considered effective.