Eduardo Harry Birgel Junior

Successful transplant of mesenchymal stem cells in induced osteonecrosis of the ovine femoral head: preliminary results.

PURPOSE Evaluate the bone tissue recovery following transplantation of ovine mesenchymal stem cells (MSC) from bone marrow and human immature dental-pulp stem cells (hIDPSC) in ovine model of induced osteonecrosis of femoral head (ONFH). METHODS Eight sheep were divided in three experimental groups. First group was composed by four animals with ONFH induced by ethanol through central decompression (CD), for control group without any treatment. The second and third group were compose by two animals, six weeks after ONFH induction received transplantation of heterologous ovine MSC (CD + oMSC), and hIDPSC (CD + hIDPSC), respectively. In both experiments the cells were transplanted without application of any type of immunosupression protocol. RESULTS Our data indicate that both cell types used in experiments were able to proliferate within injured site providing bone tissue recovery. The histological results obtained from CD+hIDPSC suggested that the bone regeneration in such animals was better than that observed in CD animals. CONCLUSION Mesenchymal stem cell transplant in induced ovine osteonecrosis of femoral head by central decompression technique is safe, and apparently favors bone regeneration of damaged tissues.

Valores de referência do leucograma de bovinos da raça Jersey criados no Estado de São Paulo

Estabeleceram-se os valores de referencia do leucograma de bovinos da raca Jersey, criados no Estado de Sao Paulo, avaliando-se a influencia exercida pelos fatores etarios em amostras de sangue colhidas de 253 femeas clinicamente sadias e nao-reagentes ao antigeno do Virus da Leucose dos Bovinos. Nas amostras de sangue, que continham EDTA como anticoagulante, foi realizada a contagem do numero total de leucocitos, em câmara de Neubauer modificada, utilizando-se o liquido de Thoma como diluidor e a contagem diferencial de leucocitos, efetuada em esfregacos sanguineos, corados pelo metodo de Rosenfeld. Demonstrou-se que o leucograma desses bovinos sofria influencia dos fatores etarios. O numero total de leucocitos e linfocitos aumentou ate 12 meses de idade, estabilizando-se entre 12 e 24 meses, para, a seguir, diminuir, progressivamente, com o desenvolvimento etario, sendo o quadro leucocitario predominantemente linfocitario. Essas variacoes foram atribuidas, principalmente, ao comportamento do numero absoluto de linfocitos. O numero absoluto do total de neutrofilos nao demonstrou variacoes significativas que pudessem ser atribuidas a influencia dos fatores etarios, mas o numero absoluto de neutrofilos com nucleo em bastonete foi maior nos bezerros com ate 6 meses de idade. O numero absoluto de eosinofilos aumentou gradativamente com o desenvolvimento etario, nao se observando variacoes significativas do numero absoluto de basofilos e monocitos. Os valores de referencia determinados para o leucograma, expressos em numeros absolutos, foram os seguintes: leucocitos- 11.847 ± 3.374 celulas/mm3; neutrofilos bastonete- 80 ± 122 celulas/mm3; neutrofilos segmentado- 2.457 ± 1.311 celulas/mm3; total de neutrofilos- 2.537 ± 1.354 celulas/mm3; eosinofilos- 335 ± 415 celulas /mm3; basofilos- 84 ± 108 celulas/mm3; linfocitos- 8.716 ± 3.028 celulas/mm3; monocitos- 174 ± 132 celulas/mm3.

Influência da gestação e do puerpério sobre o leucograma de caprinos da raça Saanen, criados no Estado de São Paulo

With the intention of evaluating the influence of pregnancy and puerperium in the leucogram of Saanen goats (Capra hircus), blood samples were taken from 150 goats bred on the State of Sao Paulo and were allotted into 5 experimental groups of 30 goats each as described: non-pregnant; initial pregnancy (30 to 60 days of pregnancy); middle pregnancy (60 to 120 days of pregnancy); late pregnancy (more than 120 days of pregnancy); kidding goats (until 30 days after parturition). The blood samples were collected with EDTA and submitted to the following tests: total leukocyte counts (in the modified Neubauer hemocytometer, using Thomas liquid as a dilute); and differential leukocyte count (made up with blood smears and stained with Rosenfeld method). The influence of pregnancy and puerperium on the leucogram was verified due to a gradual decrease on the number of leucocytes further on in pregnancy, reaching its lower values at the end of pregnancy and puerperium. This decrease occurred due to behavior observed on the absolute number of lymphocytes that also decreased further on in pregnancy. Therefore the leucograma became mostly neutrophilic at the end of pregnancy and puerperium.

Influência dos fatores raciais na função hepática de bovinos da raça Holandesa e Jersey

Para avaliar a influencia de fatores raciais sobre a funcao hepatica de bovinos foram colhidas 134 amostras de sangue, sendo 67 animais da raca Jersey e 67 da raca Holandesa, criados em Sao Paulo. No soro sanguineo realizaram-se as seguintes determinacoes: proteinograma; atividade enzimatica da aspartato aminotransferase (AST), gama glutamiltransferase (GGT), bilirrubinas indireta, direta e total. A avaliacao do proteinograma demonstrou a influencia dos fatores raciais, pois os valores medios da proteina total dos bovinos da raca Jersey (6,37±0,90 g/dl) foram menores do que os animais da raca Holandesa (6,82±0,97 g/dl).Essas variacoes foram decorrentes as fracoes proteicas que correspondem as globulinas, sendo os teores medios obtidos para bovinos da raca Jersey (3,13 ± 0,81g/dl) menores do que os encontrados para bovinos da raca Holandesa (3,73 ± 0,99 g/dl). Os valores das alfa-, beta- e gama-globulinas obtidos nos animais da raca Jersey (iguais a 0,89±0,14 g/dl; 0,71±0,14 g/dl; 2,03±0,38 g/dl) foram menores do que os encontrados para bovinos da raca Holandesa (iguais a 1,00±0,16 g/dl; 0,76±0,15 g/dl; 2,20±0,45 g/dl). A avaliacao da atividade enzimatica revelou a influencia dos fatores raciais nos resultados da AST, sendo os valores dos bovinos Jersey (49,27±17,87 U/l) maiores do que os da raca Holandesa (34,76±10,61 U/l), enquanto para a GGT nao foram observadas diferencas significativas entre os resultados da raca Jersey (19,46±33,11 U/l) e da raca Holandesa (19,08±33,26 U/l). A avaliacao dos teores sericos de bilirrubinas evidenciou variacoes sob influencia racial para os teores de bilirrubinas indireta e total, sendo os valores obtidos para a raca Jersey (0,40±0,33 mg/dl de bilirrubina indireta e 0,44±0,33 mg/dl de bilirrubina total) maiores do que os observados para a raca Holandesa (0,22±0,19 mg/dl de bilirrubina indireta e 0,25±0,19 mg/dl de bilirrubina total).

Treponemes Detected in Digital Dermatitis Lesions in Brazilian Dairy Cattle and Possible Host Reservoirs of Infection

ABSTRACT The main pathogenic treponemes causing bovine digital dermatitis were identified from 17 infected herds in southern Brazil for the first time in this study using PCR. We did not find a relationship between treponeme phylogroup composition and clinical classification. Treponema phagedenis was present in all lesions. Rumen fluid was implicated as a reservoir location for these pathogens.

Influência da gestação e do puerpério sobre o eritrograma de caprinos (Capra hircus) da raça Saanen, criados no Estado de São Paulo

The influence of pregnancy and puerperium on the erythrogram of Saanen goats (Capra hircus) was evaluate on blood samples were taken from 150 goats raised on the State of Sao Paulo and were allocated into 5 experimental groups of 30 goats each as described: non-pregnant; initial pregnancy (30 -| 60 days of pregnancy); middle pregnancy (60 -| 120 days of pregnancy); late pregnancy (more than 120 days of pregnancy); kidding goats (until 30 days after parturition). The blood samples were collected with EDTA and submitted to the following analysis: erythrocyte counts (at the modified Neubauer hemocytometer, using Gowers liquid as a dilute); packed cell volume (using the microhematocrit method); hemoglobin concentration (using cyanmethemoglobin method); calculated blood indices: MCV - mean corpuscular volume; MCH - mean corpuscular hemoglobin; MCHC - mean corpuscular hemoglobin concentration. Although lower number of erytrocytes were seen at the end of pregnancy, the blood values returned to normality during the kidding phase. These values returned to those observed in non-pregnant goats, goats in early and middle pregnancy. The evaluation of the hemoglobin concentration and packed cell volume did not show any significant variation that could be attributed to pregnancy or the puerperium. The MCV and MCH values were higher in goats in late pregnancy and during kidding.

Eritrograma de bezerras sadias, da raça Holandesa, no primeiro mês de vida

Com a finalidade de estabelecer valores de referencia e de avaliar a influencia do fator etario sobre o eritrograma de bezerras sadias, da raca Holandesa, utilizaram-se amostras de sangue de 300 animais, distribuidos por 15 grupos experimentais, de acordo com a idade, no primeiro mes de vida. As variacoes dos valores medios obtidos para os componentes do eritrograma foram as seguintes: numero de hemacias (x106/mm3) = 6,68-7,60, volume globular (%) = 29,80-33,35, taxa de hemoglobina (g/dl) = 9,00-10,43, volume corpuscular medio (fl) = 38,93 47,68, hemoglobina corpuscular media (pg) = 11,75-14,69, concentracao de hemoglobina corpuscular media (%) = 29,53-31,63% e numero de reticulocitos (x103/mm3) = 0,00-11,86. A influencia do fator etario provou-se significativa para o volume corpuscular medio, hemoglobina corpuscular media e numero de reticulocitos.

Avaliação do quadro eritrocitário e da repercussão do estado anêmico no leucograma de caprinos com verminose gastrintestinal

In order to characterize the anemia and its impact over the white blood cell count (WBC) due to gastrointestinal parasitism, cells blood count (CBC) of 96 adult Saanen goats were performed. Fifty-seven of these goats were anemic. The disease diagnosis was based on the characteristic signals of gastrointestinal parasitism (whitened conjunctival mucous, submandibular edema, loss of weight and bristling hair coat), confirmed by the presence of Trichostrongyloidea eggs in the stool test. The blood samples were collected from the jugular vein using an EDTA siliconized tubes with vacuum. Erythrocyte counts, packed cell volume, hemoglobin concentration, blood indices (MCV, MCH and MCHC), total leukocyte counts, and differential leukocyte count were performed. The goats were divided into four groups based on the magnitude of packed cell volume decrease: (1) goats without anemia, (2) mild anemia, (3) moderate anemia, and (4) severe anemia. Normocytic and normochromic anemia were observed in animals with mild to moderate anemia, while in animals with severe anemia (decreased more than half of normal values of He, Ht, Hb) hypochromic and normocytic anemia was observed. The total number of leukocytes did not change, while the anemic state due to gastrointestinal parasitism determined neutrophilia without a left shift, monocytosis and lymphopenia absolute. It was observed a change of the pattern leukocyte count, which becoming mostly neutrophilic. This reversal was more pronounced as the anemic process became more intense.

Dinâmica das proteínas séricas de fêmeas bovinas da raça holandesa naturalmente infectadas pelo vírus da leucose dos bovinos

In an attempt to determine the serum protein dynamics in cattle naturally infected with bovine leukemia virus, samples of blood sera from 60 cows of Holstein breed, raised in the State of Sao Paulo, Brazil, were collected.The animals were divided in three Groups: Group 1- was composed of 20 bovine leukemia virus antibody negative cows; Group 2 - was composed of 20 cows reacting to the antigen (gp-51) of the bovine leukemia virus without lymphocytosis and Group 3 - was composed of 20 cows reacting to the antigen (gp-51) of the bovine leukemia virus with lymphocytosis. Serum total protein was determined by the biuret reaction and serum protein fractions (alpha, beta and gammaglobulin) by electrophoresis. Immunoglobulins IgM and IgG were determined by single radial imunodiffusion.There were no signifcant differences in the serum protein profiles and the concentration of serum IgG and IgM between the three groups (bovine leukemia virus antibody negative cows; bovine leukemia virus antibody positive cows without lymphocytosis and bovine leukemia virus antibody positive cows with lymphocytosis).

Proteínas do soro lácteo de vacas da raça Jersey durante a lactação

To evaluate the whey proteins during the lactation, whey obtained from 48 milk samples collected from 12 Jersey cows before milking were studied. Cows were distributed into three groups as follows: early (30-120 days), middle (121-210 days) and end of lactation (more than 211 days). The proteinogram consisted of total proteins concentration determined by biuret method and polyacrymalide gel electrophoresis (SDS-PAGE). A gradual and significant decrease of some fractions of the whey was observed during the lactation cycle albumin, lactoferrin, immunoglobulins, β-lactoglobulin and α -lactoalbumin. The normal ranges obtained for the whey proteins of Jersey cows were: whey total proteins 569.0 to 713.0mg/dL, lactoferrin 36.0 to 49.0mg/dL, albumin 24.0 to 34.0mg/dL, immunoglobulins heavy chain 38.0 and 51.0mg/dL; immunoglobulins light chain 59.0 to 95.0mg/dL, β-lactoglobulin 207.0 to 256.0mg/dL, α-lactoalbumin 117.0 to 157.0mg/dL; protein with 226 KDa 5.80 to 12.0mg/dL, and protein with 118 KDa 2.30 to 6.80mg/dL.